Splicing: A new level of regulation in Saccharomyces cerevisiae

Linda J. Palmisano(1), Cristy L. Gelling(1), Geoffrey Kornfeld(2), Ian W. Dawes(2). (1) School of Biotech & Biomol Sci, University of New South Wales, Sydney, New South Wales, 2052, Australia; (2) Ramaciotti Centre for Gene Function Analysis and School of Biotech & Biomol Sci, University of NSW, Sydney, NSW, 2052, Australia.

During its lifespan each nuclear eukaryotic transcript will undergo a number of steps at which it may be processed in a variety of different ways. A number of mechanisms have been identified which allow the cell to vary the manner in which these post-transcriptional modifications are carried out, with potentially profound effects on the amount of protein produced in each case. This study examines the modulation of splicing as a possible additional level of post-transcriptional regulation in the model eukaryote, S. cerevisiae, and investigates the manner in which such regulation may be achieved. Spliceosomal Lsm proteins form part of an ancient family stretching back into Archaea and have been implicated in an increasing number of key RNA modification complexes in the eukaryotic cell. We studied the regulation of the Lsm proteins under a variety of metabolic conditions and examined the resulting patterns for correlation with both the splicing requirements of the cell and the amount of splicing occurring in vivo under these conditions, using a novel in vivo splicing assay. Interestingly two of the LSM genes encoding spliceosomal proteins contain introns themselves, and the extent to which these contribute to autoregulation of these genes, and their influence on the regulation of other elements of the spliceosomal complex have been explored.


Program Nr. 404B from 2004 Yeast meeting

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