Anna Ballew, Bio 55 Students, Martha Cyert, Tim
Stearns. Biological Sciences, Stanford University, 337 Campus Drive,
Stanford, CA, 94305, USA.
Bio 55 is a lab course in the Biological Sciences Department at
Stanford that aims to train a select group of students to solve
biological problems using the tools of genetics and genomics. In
this course, the students learn about and make use of the modern
toolkit of yeast genetics: the deletion collections, transcript
microarrays, ORF tag microarrays, and advanced microscopy. In the
initial part of this course we have screened the haploid yeast
deletion strain collection for mutants that are sensitive to a
variety of conditions, including 40 mM NaF, 0.01% SDS, and growth at
39 degrees. The screen was carried out using the MATa deletion
collection and all strains that are sensitive are being re-tested in
the MATalpha background as well. Based on the initial results, many
of the strains that are sensitive to these conditions were deleted
for genes involved in cell growth, metabolism and transport.
Students participating in the course will design independent
projects to follow up on findings from this screen. Final results
from the screen and the student projects will be presented.
Program Nr. 439A from 2004 Yeast meeting