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F:\Websites\www2.genetics-gsa.org\zebrafish\2014\abstracts\cgi-bin\zf14soe.pl line 262 (#1)
(W uninitialized) An undefined value was used as if it were already
defined. It was interpreted as a "" or a 0, but maybe it was a mistake.
To suppress this warning assign a defined value to your variables.
To help you figure out what was undefined, perl will try to tell you
the name of the variable (if any) that was undefined. In some cases
it cannot do this, so it also tells you what operation you used the
undefined value in. Note, however, that perl optimizes your program
and the operation displayed in the warning may not necessarily appear
literally in your program. For example, "that $foo" is usually
optimized into "that " . $foo, and the warning will refer to the
concatenation (.) operator, even though there is no . in
your program.
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Tuesday, June 24 |
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11:00 am |
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5:00 pm |
GSA Trainee Boot Camp | | Concourse Hotel |
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3:00 pm |
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9:30 pm |
Registration | | Capitol Theater |
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5:00 pm |
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6:15 pm |
Welcome Reception and Exhibits | | Overture Hall Lobby |
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5:00 pm |
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6:15 pm |
GSA Education Special Interest Group Mixer/Pedagogy Workshop | | Wisconsin Studio |
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6:15 pm |
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7:30 pm |
Plenary Session I: Emerging Technologies: Imaging, Genetics and Genomics Chair: Shannon Fisher | | Overture Hall |
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7:30 pm |
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8:30 pm |
Keynote Speaker: Sarah Tishkoff Introduction: Shannon Fisher | | Overture Hall Lobby |
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8:30 pm |
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10:30 pm |
Reception and Exhibits | | Overture Hall Lobby |
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9:00 pm |
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9:30 pm |
Organ Concert - JS Bach, Organist, Graham Lieschke | | Overture Hall |
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Wednesday, June 25 |
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7:30 am |
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5:30 pm |
Registration | | Overture Hall Lobby |
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8:30 am |
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9:45 am |
Concurrent Sessions I | | |
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Neural Degeneration, Regeneration and Disease Chair: Hiromi Hirata | | Overture Hall |
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Cancer and Growth Control Chair: James Amatruda | | Capitol Theater |
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Early Development and Patterning Chair: Bruce Draper | | Playhouse Theater |
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Metabolism Chair: Steve Farber | | Promenade Hall |
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9:45 am |
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10:45 am |
Exhibits and Coffeebreak | | Overture Hall Lobby |
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10:45 am |
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12:00 noon |
Plenary Session II: Signaling and Gene Regulation Chair: Karuna Sampath | | Overture Hall |
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12:00 noon |
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1:30 pm |
Lunch | | Concourse Hotel |
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12:00 noon |
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1:30 pm |
How to Get Published Luncheon
Hear the ins-and-outs of getting your articles published, featuring editors from the journals GENETICS and G3:Genes|Genomes|Genetics.
| | Concourse Hotel |
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1:45 pm |
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3:00 pm |
Concurrent Sessions II | | |
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Cardiovascular Development and Disease Chair: Jeroen Bakkers | | Overture Hall |
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Imaging 1 Chair: Sean Megason | | Capitol Theater |
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Neural Circuits, Neurophysiology and Behavior Chair: Stephan Neuhauss | | Playhouse Theater |
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Craniofacial and Skeletal Gene Regulation Chair: Ferenc Mueller | | Promenade Hall |
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3:00 pm |
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4:00 pm |
Exhibits and Coffeebreak | | Overture Hall Lobby |
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4:00 pm |
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6:00 pm |
Concurrent Workshops | | |
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Recombinase-Based Genome Editing and Lineage Tracing Organizers: Tom Carney and Christian Mosimann
Recombinase systems, including Cre/lox, PhiC31, and FLP/FRT, are increasingly used for advanced transgenic experiments in zebrafish. This workshop will summarize the latest reagents, approaches, and developing uses of recombinase-based modification of the genome, including applications to transgenesis, inducible gene expression, and cell lineage tracing.
The workshop will feature presentations on both individual and large-scale resources being made available to the community and also open discussion to exchange future ideas and coordinate resources/approaches. We will further hold a FAQ section addressing common protocol problems, queries and their solutions relating to Cre/LoxP and Tamoxifen induction of Cre recombination, recombination efficiency, and transgene design.
Confirmed participants:
Jim Lister, Lister Lab, Virginia Commonwealth University
Liz Perry, White Lab, Memorial Sloan Kettering Cancer Center
Albert Pan, Pan Lab, Georgia Regents University
Le Ai Trinh, Fraser Lab, University of Southern California
Yanchao Han, Poss Lab, Duke University
Lisette Madison, Chen Lab, Vanderbilt University
Michael Parsons, Leach Lab, Johns Hopkins University
| | Capitol Theater |
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ZENCODE: Zebrafish Epigenetics Organizers: Julia Horsfield and Fiona Wardle
This workshop will highlight recent zebrafish epigenetics research, and discuss the development of a zebrafish ENCODE, or ZENCODE. A panel discussion will include working towards standardised methodology for zebrafish epigenomics so that datasets from different laboratories can be combined, or compared.
4:00 pm - 4:10 pm Welcome and Introduction, Julia Horsfield and Fiona Wardle
4:10 pm - 4:30 pm Brad Cairns, Epigenomic maps of zebrafish gametes and early embryos
4:30 pm - 4:45 pm Nadine Vastenhouw, Changes in chromatin structure during genome activation
4:45 pm - 5:00 pm Ferenc Muller, Non-canonical transcriptional and post-transcriptional regulatory mechanisms during development
5:00 pm - 5:15 pm Antonio Girladez, Using ribosome foot printing to uncover the coding potential of the genome
5:15 pm - 5:35 pm Derek Stemple, Functional annotation of the zebrafish genome through systematic analysis of differential gene expression
5:35 pm - 6:00 pm Panel Discussion: integrating genomics data, genomics techniques,
progressing ZENCODE
| | Promenade Hall |
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Tools for Image Acquisition, Analysis and Increased Throughput Organizer: Rock Pulak
This workshop will discuss new tools for increasing the throughput of handling, manipulating, and orienting zebrafish larvae for image capture and some of the latest approaches for high content acquisition and analysis. The techniques discussed will cover methods for still and video images. There will be presentations on the recent advances in image acquisition and analysis in high-content screens including commercial products, such as Lightsheet microscopy (Zeiss), examples of image analysis tools (commercial and open source software), and the VAST BioImager instrumentation (Union Biometrica). Presenters will be from academic labs utilizing the increased throughput afforded by these technologies in areas such as fundamental phenotyping, drug screening and toxicology.
Speakers:
Karl J. Clark, Mayo Clinic, Lightsheet microscopy in toxicology studies with zebrafish larvae.
Erica Davis/Christelle Golzio, Duke University, Modeling pediatric genetic disorders in zebrafish: Accelerated throughput with VAST BioImager.
Peter Eimon, MIT, New technologies for quantitative phenotyping of zebrafish larvae.
| | Playhouse Theater |
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4:00 pm |
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6:00 pm |
GSA Plenary Session and Workshop for Undergraduate Researchers | | Wisconsin Studio |
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6:00 pm |
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8:00 pm |
Dinner | | Memorial Union |
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6:00 pm |
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8:00 pm |
Undergraduate Dinner | | Capitol View |
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8:00 pm |
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11:00 pm |
Poster Session 1 and Exhibits 8:00 pm - 9:15 pm Presenters of all even session I posters 9:15 pm - 10:30 pm Presenters of all odd session I posters 10:30 pm - 11:00 pm Open Viewing | | Memorial Union |
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Thursday, June 26 |
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8:00 am |
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5:30 pm |
Registration | | Overture Hall Lobby |
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8:30 am |
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9:45 am |
Plenary Session III: Regeneration and Stem Cells Chair: Tatjana Piotrowski | | Overture Hall |
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9:45 am |
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10:45 am |
Exhibits and Coffeebreak | | Overture Hall Lobby |
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10:45 am |
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12:00 noon |
Concurrent Sessions III | | |
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Imaging 2 Chair: Nadine Peyrieras | | Overture Hall |
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Morphogenesis and Cell Migration Chair: Holger Knaut | | Capitol Theater |
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Chemical Biology Chair: Elizabeth Patton | | Playhouse Theater |
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Evolution Chair: David Parichy | | Promenade Hall |
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12:00 noon |
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1:30 pm |
Lunch | | Concourse Hotel |
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12:00 noon |
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1:30 pm |
GSA Career Luncheon
The GSA Career Luncheon is an excellent opportunity for undergraduates, graduate students, and postdoctoral fellows to have informal conversations with senior career scientists regarding the unique challenges and rewards of a scientific career. The luncheon is organized by topic table. Topics may include things like: transition to independence, work-family balance, teaching at undergraduate institutions, careers outside traditional academia, the job search, the postdoc search, choosing the right graduate advisor, etc.
| | Concourse Hotel |
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1:30 pm |
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2:30 pm |
Keynote Speaker: John Postlethwait Introduction: Tom Schilling | | Overture Hall |
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2:30 pm |
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3:00 pm |
Chi-Bin Chien Award Presentation
Introduction: David Grunwald | | Overture Hall |
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3:00 pm |
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4:00 pm |
Exhibits and Coffeebreak | | Overture Hall Lobby |
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4:00 pm |
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6:00 pm |
Concurrent Workshops | | |
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Emerging Tools and Methods for Quantitative Imaging of Vascular Development Organizers: Ben Hogan and Shane Herbert
This workshop will highlight new approaches in the analysis of the zebrafish vasculature with a particular focus on the visualization and quantification of dynamic cellular processes during vascular development. Emphasis will be placed on utilization of advanced real-time imaging approaches that push the current boundaries of cellular and temporal resolution. In particular, there will be a focus on the integration of imaging studies with computational modeling and novel analytical methods to provide a quantitative interpretation of vessel phenotypes. In addition, there will be discussion from speakers about the commonly used tools in the field. These tools, as well as the emerging techniques discussed above, all promise to be broadly applicable in vascular cell biological studies in zebrafish.
Topics include: Advanced real-time imaging techniques, New analytical tools and computational methods for the quantification of angiogenic phenotypes, Sub-cellular resolution imaging of vascular organelle dynamics, achieving phenotypic specificity in analysis of vascular phenotypes.
Confirmed Speakers:
Heinz-Georg Belting (Affolter Lab), University of Basel, Switzerland
Emily Steed (Vermot Lab), IGBMC, France
Katie Bentley, Harvard University, USA
Nathan Lawson, University of Massachusetts, USA
Shane Herbert, University of Manchester, UK
| | Playhouse Theater |
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Practical Approaches to Using Zebrafish in Education
Organizers: Jennifer Bonner and Suzanne Gribble
Using zebrafish for educational purposes? Our time will be spent engaging in large and small group discussions on the topics of teaching students to read scientific literature, outreach program development, and how to lead undergraduate research students through the process of designing, implementing, analyzing and presenting research projects while accomplishing your teaching and research objectives.
Confirmed participants:
Nathalia Holtzman, Queens University of New York City
Steven Farber, Carnegie Institute for Science
| | Wisconsin Studio |
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Post-embryonic development: expanding human disease models and understanding variation
Organizer: Matthew Harris
Zebrafish is quickly becoming the go to model for analysis of gene function during development and in assessing genetic variants associated with human disease. Post-embryonic development is becoming an important focus of research as beginning studies point to the utility of late phenotypes in disease modeling as well as to understand variation in form and physiology. However, there remains a lack of a unified discussion of the questions/frontiers in the field and what tools are needed to take advantage of the zebrafish to address these questions.
The workshop is designed to bring together researchers that have focused on mechanisms of late development to discuss important aspects of organogenesis and physiology that are uncovering novel aspects of gene function and providing important models for disease. Topics will center on particular organ systems or processes to highlight the current state of the field, where the field is going, as well as developing specific tools for analysis. The overall goal of the workshop is to establish common goals and efforts to promote use of adult zebrafish in modeling disease and understanding variation among vertebrates.
Confirmed speakers:
Development of Skeleton:
Matthew Harris, Harvard Medical School; Boston Childrens Hospital
Shannon Fisher, University of Pennsylvania
Adipogenesis and Gut Microbial dynamics:
John Rawls, Duke University
Adult neurogenesis and behavior:
Hitoshi Okamoto, RIKEN Brain Science Institute
Reproduction:
Kellee Siegfried, University of Massachusetts Boston
Bruce Draper, University of California Davis
Life History Regulation: Metamorphosis:
David Parichy, University of Washington
Aging and senescence:
Matthew Harris, Harvard Medical School; Boston Childrens Hospital
| | Promenade Hall |
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6:00 pm |
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8:00 pm |
Dinner | | Memorial Union |
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8:00 pm |
- |
11:00 pm |
Poster Session 2 and Exhibits 8:00 pm - 9:15 pm Presenters of all even session II posters 9:15 pm - 10:30 pm Presenters of all odd session II posters 10:30 pm - 11:00 pm Open Viewing | | Memorial Union |
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Friday, June 27 |
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8:00 am |
- |
4:00 pm |
Registration | | Overture Hall Lobby |
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8:30 am |
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9:45 am |
Concurrent Sessions IV | | |
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Gene Regulation and Epigenetics Chair: Antonio Giraldez | | Overture Hall |
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Neural Development Chair: Rich Dorsky | | Capitol Theater |
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Cilia, Cell and Tissue Polarity Chair: Rebecca Burdine | | Playhouse Theater |
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Infection and Immunity Chair: David Tobin | | Promenade Hall |
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9:45 am |
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10:45 am |
Exhibits and Coffeebreak | | Overture Hall Lobby |
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10:45 am |
- |
12:00 noon |
Plenary Session IV: Physiology and Disease Chair: Graham Lieschke | | Overture Hall |
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12:00 noon |
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1:00 pm |
Community Meeting Lorette Javois, NIH, funding opportunities ZFIN/ZIRC updates | | Overture Hall |
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12:30 pm |
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2:00 pm |
Lunch | | Concourse Hotel |
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1:00 pm |
- |
2:00 pm |
Advocacy Luncheon
Staff members from the FASEB Office of Public Affairs will discuss the latest policy and legislative news affecting funding for NIH, describe why it is important for scientists to speak out about issues that affect their grants and research, and share tips for engaging in advocacy.
| | Concourse Hotel |
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2:00 pm |
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4:00 pm |
Concurrent Workshops | | |
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Cancer in the Zebrafish: moving from promise to achievement Organizers: Jason Berman and David Langenau, Richard White
The zebrafish has emerged as a powerful model for human disease over the past decade, but still remains at the periphery of most mainstream disease studies, particularly in cancer. The purpose of this workshop is to give an overview of several major technologies available in the zebrafish (both strengths and limitations), and how these present unique opportunities to address problems in cancer biology. In addition to the talks, we will leave time for a discussion of strategies to propel the zebrafish into a prominent place in cancer modeling.
Speakers:
Rich White, A brief introduction to cancer modeling in the zebrafish
Rich White, Genomics Approaches (DNA/RNAseq)
Yariv Houvras, Epigenetics
Jane Zhu, Functional Genomics
Joanna Yeh, Chemical Genetics
David Langenau, Allograft Transplantation
Jason Berman and Herman Spaink, Xenograft Transplantation
| | Overture Hall |
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Emerging Techniques in Neural Circuit Analysis Organizers: Filippo del Bene and Marnie Halpern
With recent progress in live brain imaging, the zebrafish is a superior model for correlating neural activity with behavior. The workshop "Emerging techniques in neural circuit analysis" will present new technologies for the analysis of neural circuit development and function. Participants will discuss potential problems and successful attempts to apply new transgenic tools such as genetically encoded voltage and calcium indicators, optogenetic probes, brainbow and transynaptic labeling, and novel behavioral paradigms. The goal is to coordinate the efforts of multiple labs in adapting state-of-the-art approaches to zebrafish, by highlighting recent advances and addressing unresolved challenges.
Speaker list:
Erik Duboué, Carnegie, Quantifying the response to an aversive stimulus in larval zebrafish
Claire Wyart, ICM, France, Channel Rhodopsin mediated mapping of connectivity in the spinal cord of zebrafish larva
Douglas Kim, Janelia, Optimized fluorescent proteins for imaging neural activity
Hitoshi Okamoto, Riken, Japan, Optogenetics in adult zebrafish
Koichi Kawakami, NIG, Japan, Genetic dissection of the adult zebrafish brain by the Gal4-UAS method
Misha Ahrens, Janelia, Whole-brain functional maps
Kurt Marsden, Univ. of Penn, Mapping neural circuits underlying startle modulation in head restrained zebrafish larvae
David Prober, Caltech, Using TRP channels to activate and ablate neurons
Alex Schier, Harvard, An immunohistochemical method to detect neuronal activity
Harold Burgess, NIH, New transgenic tools for manipulating neuronal circuitry
| | Capitol Theater |
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Maternally Controlled Development - Current Limitations of Analysis and Future Perspectives Organizers: Sreelaja Nair and Roland Dosch
Early development of all eukaryotic embryos relies on factors loaded into the egg by the female prior to fertilization. Dissecting their roles poses several technical challenges primarily in the isolation of critical maternal factors, the visualization of their dynamic cellular localization and analyzing their biochemical function. Although zebrafish emerged as an outstanding vertebrate for the analysis of maternal factors, our understanding of their function is mostly limited to forward genetic mutation approaches. The workshop is intended to share through short talks, innovations under development to broaden the methodologies that can be used to effectively study maternal genes. The goal of the subsequent discussion is to elaborate on efforts to circumvent technical limitations and resource sharing so that zebrafish continues to excel in all aspects of research on maternally controlled development.
Short Talks
Francisco Pelegri, Genetic approaches to isolate maternal genes
Roland Dosch, Protein knockdown to investigate maternal factors
Sreelaja Nair, Oocyte culture methods and targeted isolation of maternal factors
Karuna Sampath, Packing and moving: localized transport in oocytes and early embryos
| | Promenade Hall |
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Digestive System Biology and Disease
Organizers: Wolfram Goessling and John Rawls
This workshop will bring together the growing community of researchers using the zebrafish to investigate digestive organ development, physiology, and disease. The format of this workshop will consist of a series of short presentations and community discussions about emerging technologies, resources, needs, funding strategies, and other topics of specific relevance to this community.
2:00 pm Michel Bagnat, Duke University, Genetic and cell biological approaches to study digestive tract development and function in zebrafish
2:25 pm Andy Cox, Brigham and Women''s Hospital, ,Zebrafish models of liver injury and their possible translational application
2:50 pm Stephen Watts, University of Alabama at Birmingham, Zebrafish nutrition: opportunities lost
3:15 pm John Rawls, Duke University, Factors influencing microbiota assembly in the zebrafish intestine
3:40 pm Steven Farber, Carnegie Institution, Involvement of the digestive system research community in Zebrafish Disease Models
| | Wisconsin Studio |
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4:30 pm |
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7:30 pm |
Poster Session 3 and Exhibits 4:30 pm - 5:45 pm Presenters of all even session III posters 5:45 pm - 7:00 pm Presenters of all odd session III posters 7:00 pm - 7:30 pm Open Viewing | | Memorial Union |
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7:30 pm |
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9:00 pm |
Banquet Buffet | | Memorial Union |
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9:00 pm |
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1:00 am |
Conference Party featuring Natty Nation on the Union Terrace and a DJ, Mike Carlson, in Tripp Commons | | Memorial Union |
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Saturday, June 28 |
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9:00 am |
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10:15 am |
Concurrent Sessions V | | |
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Genetics and Genomics Chair: Brad Cairns | | Overture Hall |
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Hematopoiesis Chair: Zilong Wen | | Capitol Theater |
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Sensory Systems Chair: Teresa Nicolson | | Promenade Hall |
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Endodermal and Mesodermal Organs Chair: Joan Heath | | Wisconsin Studio |
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10:15 am |
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10:45 am |
Coffeebreak | | Overture Hall Lobby |
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10:45 am |
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12:00 noon |
Plenary Session V: Morphogenesis and Organogenesis Chair: Tom Schilling | | Overture Hall |
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11:30 am |
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12:00 noon |
Box Lunches will be available and shuttle busses for rides to the airport | | Overture Hall Lobby |
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Use of uninitialized value $data in print at
F:\Websites\www2.genetics-gsa.org\zebrafish\2014\abstracts\cgi-bin\zf14soe.pl line 269 (#1)